Application note: Monitoring receptor ligand binding in living cells In this application we will show how a cAMP BRET biosensor can be used to monitor ligand binding with the help of the CLARIOstar microplate reader… A number of assay techniques are used to study ligand binding to GPCRs in living cells. The most well-known being the use of radioactively labelled …

Corals’ pH Sensor Identified Soluble adenylyl cyclase measures and responds to pH changes in coral cells, but whether it can help the animals withstand ocean acidification is not yet known. Researchers have identified an enzyme that helps corals keep a steady pH in their cells, even as the acidity changes in their environments. Corals experience pH variation due to fluctuations …