The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research fields including algal, plant, and ciliary biology. Accordingly, decades-long standing inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research. Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins with single-stranded DNA repair templates results in precise and targeted DNA replacement with as much as ∼۱۰% efficiency in …

If green algae of the species Chlamydomonas reinhardtii meet Pseudomonas protegens bacteria, their fate is sealed. The bacteria, measuring only some two micrometres, surround the algae, which are around five times larger, and attack them with a deadly toxic cocktail. The algae lose their flagella, which renders them immobile. The green single-celled organisms then become deformed and are no longer …

Fueling the future: Scientists promote new method of algal hydrogen production Changing the way the nation generates and consumes energy is at the heart of a new NSF grant awarded to Arizona State University and Kevin Redding, professor in the School of Molecular Sciences and director of the Center for Bioenergy and Photosynthesis (CB&P). The goal of Redding and his …